Everything that attacks the colony in Wave Defence, the weekly boss and Nest Siege. Traits change how they fight and which card bonuses counter them.
| Enemy | Family | First age | Trait | Role |
|---|---|---|---|---|
| Fruit fly | Flies (Diptera) | Stone Age | Swarm | Normal |
| Springtail | Springtails (Collembola) | Stone Age | Fast | Normal |
| Silverfish pack | Silverfish (Zygentoma) | Stone Age | Fast | Elite |
| Dust mite | Spiders and mites (Arachnida) | Stone Age | Swarm | Normal |
| Earwig | Earwigs (Dermaptera) | Stone Age | Armoured | Normal |
| Daddy long-legs spider | Spiders and mites (Arachnida) | Stone Age | Venom | Boss |
| Slater | Slaters (Isopoda) | Stone Age | Armoured | Normal |
| Rival ant scout | Rival ants | Stone Age | Thief | Normal |
| Rival ant raid | Rival ants | Stone Age | Swarm | Elite |
| House centipede (young) | Centipedes and millipedes (Myriapoda) | Stone Age | Fast | Normal |
| Rival colony's soldier guard | Rival ants | Stone Age | Armoured | Boss |
| Ladybird beetle | Beetles (Coleoptera) | Bronze Age | Armoured | Normal |
| Rove beetle | Beetles (Coleoptera) | Bronze Age | Fast | Normal |
| Jumping spider | Spiders and mites (Arachnida) | Bronze Age | Fast | Elite |
| Wolf spider | Spiders and mites (Arachnida) | Bronze Age | Venom | Boss |
| Cricket | Crickets and grasshoppers (Orthoptera) | Iron Age | Fast | Normal |
| Cockroach nymph | Cockroaches and termites (Blattodea) | Iron Age | Swarm | Normal |
| Rival fire-ant raid | Rival ants | Iron Age | Venom | Elite |
| Huntsman spider | Spiders and mites (Arachnida) | Iron Age | Fast | Boss |
| Silverfish swarm | Silverfish (Zygentoma) | Steam Age | Swarm | Normal |
| Moth | Moths (Lepidoptera) | Steam Age | Flying | Normal |
| Cockroach | Cockroaches and termites (Blattodea) | Steam Age | Armoured | Elite |
| House centipede | Centipedes and millipedes (Myriapoda) | Steam Age | Fast | Boss |
| Cockroach army | Cockroaches and termites (Blattodea) | Industrial Age | Swarm | Elite |
| House fly | Flies (Diptera) | Industrial Age | Flying | Normal |
| Slater horde | Slaters (Isopoda) | Industrial Age | Armoured | Normal |
| House mouse | Mammal | Industrial Age | Thief | Boss |
| European wasp | Wasps and bees (Hymenoptera) | Atomic Age | Flying | Normal |
| Praying mantis | Mantises (Mantodea) | Atomic Age | Armoured | Elite |
| MutANT swarm | Rival ants | Atomic Age | Venom | Normal |
| Giant praying mantis | Mantises (Mantodea) | Atomic Age | Armoured | Boss |
| Argentine supercolony | Rival ants | Information Age | Swarm | Elite |
| Robotic pest | Machines | Information Age | Armoured | Normal |
| Drone swarm | Machines | Information Age | Flying | Normal |
| The exterminator's machine | Machines | Information Age | Armoured | Boss |
| Alien insect | Alien | Space Age | Venom | Normal |
| Meteor mite | Alien | Space Age | Swarm | Normal |
| Space pirate beetles | Alien | Space Age | Thief | Elite |
| The Void Beetle | Alien | Space Age | Armoured | Boss |
| Shadow colony | Shadow | Singularity | Swarm | Normal |
| Entropy swarm | Shadow | Singularity | Venom | Elite |
| Satant's Champion | Shadow | Singularity | Armoured | Boss |
| Stink bug | True bugs (Hemiptera) | Bronze Age | Armoured | Normal |
| Antlion | Lacewings (Neuroptera) | Iron Age | Fast | Normal |
| Flea swarm | Fleas (Siphonaptera) | Iron Age | Swarm | Normal |
| Dragonfly | Dragonflies (Odonata) | Steam Age | Flying | Normal |
| Stick insect | Stick insects (Phasmatodea) | Industrial Age | Armoured | Normal |